Functional analysis of long-chain n-alkane degradation by Dietzia spp.
نویسندگان
چکیده
منابع مشابه
Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. E1.
The newly isolated strain E1, identified as a Dietzia sp., proved to have an excellent ability to degrade n-C12 to n-C38 alkane components of crude oil. The preferred substrate was the very long-chain alkane n-eicosane at an optimal temperature of 37 degrees C and an optimal pH of 8 under aerobic conditions. The growth and substrate uptake kinetics were monitored during the n-alkane fermentatio...
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Acinetobacter sp. strain DSM 17874 is capable of utilizing n-alkanes with chain lengths ranging from that of decane (C10H22) to that of tetracontane (C40H82) as a sole carbon source. Two genes encoding AlkB-type alkane hydroxylase homologues, designated alkMa and alkMb, have been shown to be involved in the degradation of n-alkanes with chain lengths of from 10 to 20 C atoms in this strain. Her...
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BACKGROUND Low catalytic activities of pathway enzymes are often a limitation when using microbial based chemical production. Recent studies indicated that the enzyme activity of aldehyde decarbonylase (AD) is a critical bottleneck for alkane biosynthesis in Saccharomyces cerevisiae. We therefore performed functional screening to identify efficient ADs that can improve alkane production by S. c...
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Upon growth on n-hexadecane (C(16)), n-tetracosane (C(24)), and n-hexatriacontane (C(36)), Dietzia sp. strain DQ12-45-1b could produce different glycolipids, phospholipids, and lipopeptides. Interestingly, cultivation with C(36) increased cell surface hydrophobic activity, which attenuated the negative effect of the decline of the emulsification activity. These results suggest that the mechanis...
متن کاملMetabolic and stress responses of Acinetobacter oleivorans DR1 during long‐chain alkane degradation
Acinetobacter oleivorans DR1 can utilize C12 -C30 alkanes as a sole carbon source but not short-chain alkanes (C6 , C10 ). Two copies of each alkB-, almA- and ladA-type alkane hydroxylase (AH) are present in the genome of DR1 cells. Expression and mutational analyses of AHs showed that alkB1 and alkB2 are the major AH-encoding genes under C12 -C30 , and the roles of other almA- and ladA genes a...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2011
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.2010.02198.x